Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications
- Publikationstyp:
- Zeitschriftenaufsatz
- Metadaten:
-
- Autoren
- Elena Stengelin
- Brice Nzigou Mombo
- Mihail Mondeshki
- Guillermo L Beltramo
- Martin A Lange
- Patrick Schmidt
- Hajo Frerichs
- Serafine V Wegner
- Sebastian Seiffert
- Autoren-URL
- https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000680490200001&DestLinkType=FullRecord&DestApp=WOS_CPL
- DOI
- 10.1002/mabi.202100209
- eISSN
- 1616-5195
- Externe Identifier
- Clarivate Analytics Document Solution ID: UR6SB
- PubMed Identifier: 34342150
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- MACROMOLECULAR BIOSCIENCE
- Schlüsselwörter
- 3R principle
- microfluidics
- microgels
- microtissue engineering
- stimuli-responsive polymers
- polydopamine surface coating
- Artikelnummer
- ARTN 2100209
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Titel
- Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications
- Sub types
- Article
- Ausgabe der Zeitschrift
- 21
Datenquelle: Web of Science (Lite)
- Andere Metadatenquellen:
-
- Abstract
- <jats:title>Abstract</jats:title><jats:p>Functional microgels provide a versatile basis for synthetic in vitro platforms as alternatives to animal experiments. The tuning of the physical, chemical, and biological properties of synthetic microgels can be achieved by blending suitable polymers and formulating them such to reflect the heterogenous and complex nature of biological tissues. Based on this premise, this paper introduces the development of volume‐switchable core–shell microgels as 3D templates to enable cell growth for microtissue applications, using a systematic approach to tune the microgel properties based on a deep conceptual and practical understanding. Microscopic microgel design, such as the tailoring of the microgel size and spherical shape, is achieved by droplet‐based microfluidics, while on a nanoscopic scale, a thermoresponsive polymer basis, poly(<jats:italic>N</jats:italic>‐isopropylacrylamide) (PNIPAAm), is used to provide the microgel volume switchability. Since PNIPAAm has only limited cell‐growth promoting properties, the cell adhesion on the microgel is further improved by surface modification with polydopamine, which only slightly affects the microgel properties, thereby simplifying the system. To further tune the microgel thermoresponsiveness, different amounts of <jats:italic>N</jats:italic>‐hydroxyethylacrylamide are incorporated into the PNIPAAm network. In a final step, cell growth on the microgel surface is investigated, both at a single microgel platform and in spheroidal cell structures.</jats:p>
- Autoren
- Elena Stengelin
- Brice Nzigou Mombo
- Mihail Mondeshki
- Guillermo L Beltramo
- Martin A Lange
- Patrick Schmidt
- Hajo Frerichs
- Seraphine V Wegner
- Sebastian Seiffert
- DOI
- 10.1002/mabi.202100209
- eISSN
- 1616-5195
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Macromolecular Bioscience
- Sprache
- en
- Online publication date
- 2021
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Herausgeber
- Wiley
- Herausgeber URL
- http://dx.doi.org/10.1002/mabi.202100209
- Datum der Datenerfassung
- 2023
- Titel
- Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications
- Ausgabe der Zeitschrift
- 21
Datenquelle: Crossref
- Abstract
- Functional microgels provide a versatile basis for synthetic in vitro platforms as alternatives to animal experiments. The tuning of the physical, chemical, and biological properties of synthetic microgels can be achieved by blending suitable polymers and formulating them such to reflect the heterogenous and complex nature of biological tissues. Based on this premise, this paper introduces the development of volume-switchable core-shell microgels as 3D templates to enable cell growth for microtissue applications, using a systematic approach to tune the microgel properties based on a deep conceptual and practical understanding. Microscopic microgel design, such as the tailoring of the microgel size and spherical shape, is achieved by droplet-based microfluidics, while on a nanoscopic scale, a thermoresponsive polymer basis, poly(N-isopropylacrylamide) (PNIPAAm), is used to provide the microgel volume switchability. Since PNIPAAm has only limited cell-growth promoting properties, the cell adhesion on the microgel is further improved by surface modification with polydopamine, which only slightly affects the microgel properties, thereby simplifying the system. To further tune the microgel thermoresponsiveness, different amounts of N-hydroxyethylacrylamide are incorporated into the PNIPAAm network. In a final step, cell growth on the microgel surface is investigated, both at a single microgel platform and in spheroidal cell structures.
- Addresses
- Department of Chemistry, Johannes Gutenberg-University Mainz, Mainz, D-55128, Germany.
- Autoren
- Elena Stengelin
- Brice Nzigou Mombo
- Mihail Mondeshki
- Guillermo L Beltramo
- Martin A Lange
- Patrick Schmidt
- Hajo Frerichs
- Seraphine V Wegner
- Sebastian Seiffert
- DOI
- 10.1002/mabi.202100209
- eISSN
- 1616-5195
- Externe Identifier
- PubMed Identifier: 34342150
- Funding acknowledgements
- European Research Council: ARTIST (# 757593)
- Deutsche Forschungsgemeinschaft:
- Open access
- false
- ISSN
- 1616-5187
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Macromolecular bioscience
- Schlüsselwörter
- Animals
- Indoles
- Polymers
- Gels
- Microgels
- Sprache
- eng
- Medium
- Print-Electronic
- Online publication date
- 2021
- Paginierung
- e2100209
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Publisher licence
- CC BY
- Datum der Datenerfassung
- 2021
- Titel
- Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications.
- Sub types
- Research Support, Non-U.S. Gov't
- Journal Article
- Ausgabe der Zeitschrift
- 21
Datenquelle: Europe PubMed Central
- Abstract
- Functional microgels provide a versatile basis for synthetic in vitro platforms as alternatives to animal experiments. The tuning of the physical, chemical, and biological properties of synthetic microgels can be achieved by blending suitable polymers and formulating them such to reflect the heterogenous and complex nature of biological tissues. Based on this premise, this paper introduces the development of volume-switchable core-shell microgels as 3D templates to enable cell growth for microtissue applications, using a systematic approach to tune the microgel properties based on a deep conceptual and practical understanding. Microscopic microgel design, such as the tailoring of the microgel size and spherical shape, is achieved by droplet-based microfluidics, while on a nanoscopic scale, a thermoresponsive polymer basis, poly(N-isopropylacrylamide) (PNIPAAm), is used to provide the microgel volume switchability. Since PNIPAAm has only limited cell-growth promoting properties, the cell adhesion on the microgel is further improved by surface modification with polydopamine, which only slightly affects the microgel properties, thereby simplifying the system. To further tune the microgel thermoresponsiveness, different amounts of N-hydroxyethylacrylamide are incorporated into the PNIPAAm network. In a final step, cell growth on the microgel surface is investigated, both at a single microgel platform and in spheroidal cell structures.
- Autoren
- Elena Stengelin
- Brice Nzigou Mombo
- Mihail Mondeshki
- Guillermo L Beltramo
- Martin A Lange
- Patrick Schmidt
- Hajo Frerichs
- Seraphine V Wegner
- Sebastian Seiffert
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/34342150
- DOI
- 10.1002/mabi.202100209
- eISSN
- 1616-5195
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Macromol Biosci
- Schlüsselwörter
- 3R principle
- microfluidics
- microgels
- microtissue engineering
- polydopamine surface coating
- stimuli-responsive polymers
- Animals
- Gels
- Indoles
- Microgels
- Polymers
- Sprache
- eng
- Country
- Germany
- Paginierung
- e2100209
- Datum der Veröffentlichung
- 2021
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Titel
- Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications.
- Sub types
- Journal Article
- Research Support, Non-U.S. Gov't
- Ausgabe der Zeitschrift
- 21
Datenquelle: PubMed
- Author's licence
- CC-BY
- Autoren
- Elena Stengelin
- Brice Nzigou Mombo
- Mihail Mondeshki
- Guillermo L Beltramo
- Martin A Lange
- Patrick Schmidt
- Hajo Frerichs
- Seraphine V Wegner
- Sebastian Seiffert
- Hosting institution
- Universitätsbibliothek Mainz
- Sammlungen
- JGU-Publikationen
- Resource version
- Published version
- DOI
- 10.1002/mabi.202100209
- File(s) embargoed
- false
- Open access
- true
- ISSN
- 1616-5195
- Ausgabe der Veröffentlichung
- 9
- Zeitschrift
- Macromolecular bioscience
- Schlüsselwörter
- 540 Chemie
- 540 Chemistry and allied sciences
- Sprache
- eng
- Open access status
- Open Access
- Paginierung
- 2100209
- Datum der Veröffentlichung
- 2021
- Public URL
- https://openscience.ub.uni-mainz.de/handle/20.500.12030/8079
- Herausgeber
- Wiley-VCH
- Datum der Datenerfassung
- 2022
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2022
- Zugang
- Public
- Titel
- Rational design of thermoresponsive microgel templates with polydopamine surface coating for microtissue applications
- Ausgabe der Zeitschrift
- 21
Files
rational_design_of_thermoresp-20221017110951449.pdf
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