Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation

Publikationstyp:
Zeitschriftenaufsatz
Metadaten:
Autoren
  • Alexandra Brahmer
  • Elmo Neuberger
  • Leona Esch-Heisser
  • Nils Haller
  • Malene Moeller Jorgensen
  • Rikke Baek
  • Wiebke Moebius
  • Perikles Simon
  • Eva-Maria Kraemer-Albers
Autoren-URL
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=fis-test-1&SrcAuth=WosAPI&KeyUT=WOS:000469231700001&DestLinkType=FullRecord&DestApp=WOS_CPL
DOI
10.1080/20013078.2019.1615820
Externe Identifier
  • Clarivate Analytics Document Solution ID: IA0FG
  • PubMed Identifier: 31191831
ISSN
2001-3078
Ausgabe der Veröffentlichung
1
Zeitschrift
JOURNAL OF EXTRACELLULAR VESICLES
Schlüsselwörter
  • Extracellular vesicles
  • exosomes
  • exercise
  • plasma
  • size exclusion chromatography
  • immunobead isolation
  • multiplex phenotyping
Artikelnummer
ARTN 1615820
Datum der Veröffentlichung
2019
Status
Published
Titel
Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation
Sub types
  • Article
Ausgabe der Zeitschrift
8

Datenquelle: Web of Science (Lite)

Andere Metadatenquellen:
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>Physical activity initiates a wide range of multi‐systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise‐associated adaptive systemic signalling. Circulating EVs comprise a heterogeneous collection of different EV‐subclasses released from various cell types. So far, a comprehensive picture of the parental and target cell types, EV‐subpopulation diversity and functional properties of EVs released during exercise (ExerVs) is lacking. Here, we performed a detailed EV‐phenotyping analysis to explore the cellular origin and potential subtypes of ExerVs.</jats:p><jats:p>Healthy male athletes were subjected to an incremental cycling test until exhaustion and blood was drawn before, during, and immediately after the test. Analysis of total blood plasma by EV Array suggested endothelial and leukocyte characteristics of ExerVs. We further purified ExerVs from plasma by size exclusion chromatography as well as CD9‐, CD63‐ or CD81‐immunobead isolation to examine ExerV‐subclass dynamics. EV‐marker analysis demonstrated increasing EV‐levels during cycling exercise, with highest levels at peak exercise in all EV‐subclasses analysed. Phenotyping of ExerVs using a multiplexed flow‐cytometry platform revealed a pattern of cell surface markers associated with ExerVs and identified lymphocytes (CD4, CD8), monocytes (CD14), platelets (CD41, CD42, CD62P), endothelial cells (CD105, CD146) and antigen presenting cells (MHC‐II) as ExerV‐parental cells. We conclude that multiple cell types associated with the circulatory system contribute to a pool of heterogeneous ExerVs, which may be involved in exercise‐related signalling mechanisms and tissue crosstalk.</jats:p>
Autoren
  • Alexandra Brahmer
  • Elmo Neuberger
  • Leona Esch‐Heisser
  • Nils Haller
  • Malene Moeller Jorgensen
  • Rikke Baek
  • Wiebke Möbius
  • Perikles Simon
  • Eva‐Maria Krämer‐Albers
DOI
10.1080/20013078.2019.1615820
eISSN
2001-3078
ISSN
2001-3078
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of Extracellular Vesicles
Sprache
en
Online publication date
2019
Datum der Veröffentlichung
2019
Status
Published
Herausgeber
Wiley
Herausgeber URL
http://dx.doi.org/10.1080/20013078.2019.1615820
Datum der Datenerfassung
2023
Titel
Platelets, endothelial cells and leukocytes contribute to the exercise‐triggered release of extracellular vesicles into the circulation
Ausgabe der Zeitschrift
8

Datenquelle: Crossref

Abstract
Physical activity initiates a wide range of multi-systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise-associated adaptive systemic signalling. Circulating EVs comprise a heterogeneous collection of different EV-subclasses released from various cell types. So far, a comprehensive picture of the parental and target cell types, EV-subpopulation diversity and functional properties of EVs released during exercise (ExerVs) is lacking. Here, we performed a detailed EV-phenotyping analysis to explore the cellular origin and potential subtypes of ExerVs. Healthy male athletes were subjected to an incremental cycling test until exhaustion and blood was drawn before, during, and immediately after the test. Analysis of total blood plasma by EV Array suggested endothelial and leukocyte characteristics of ExerVs. We further purified ExerVs from plasma by size exclusion chromatography as well as CD9-, CD63- or CD81-immunobead isolation to examine ExerV-subclass dynamics. EV-marker analysis demonstrated increasing EV-levels during cycling exercise, with highest levels at peak exercise in all EV-subclasses analysed. Phenotyping of ExerVs using a multiplexed flow-cytometry platform revealed a pattern of cell surface markers associated with ExerVs and identified lymphocytes (CD4, CD8), monocytes (CD14), platelets (CD41, CD42, CD62P), endothelial cells (CD105, CD146) and antigen presenting cells (MHC-II) as ExerV-parental cells. We conclude that multiple cell types associated with the circulatory system contribute to a pool of heterogeneous ExerVs, which may be involved in exercise-related signalling mechanisms and tissue crosstalk.
Addresses
  • Institute of Developmental Biology and Neurobiology, Biology of Extracellular Vesicles, University of Mainz, Mainz, Germany.
Autoren
  • Alexandra Brahmer
  • Elmo Neuberger
  • Leona Esch-Heisser
  • Nils Haller
  • Malene Moeller Jorgensen
  • Rikke Baek
  • Wiebke Möbius
  • Perikles Simon
  • Eva-Maria Krämer-Albers
DOI
10.1080/20013078.2019.1615820
eISSN
2001-3078
Externe Identifier
  • PubMed Identifier: 31191831
  • PubMed Central ID: PMC6542154
Funding acknowledgements
  • Boehringer Ingelheim Stiftung: DRZ A 17
  • Johannes Gutenberg University Mainz: Stufe1
  • Boehringer Ingelheim Foundation: DRZ A 17
  • Johannes Gutenberg-Universität Mainz: Stufe 1
Open access
true
ISSN
2001-3078
Ausgabe der Veröffentlichung
1
Zeitschrift
Journal of extracellular vesicles
Sprache
eng
Medium
Electronic-eCollection
Online publication date
2019
Open access status
Open Access
Paginierung
1615820
Datum der Veröffentlichung
2019
Status
Published
Publisher licence
CC BY-NC
Datum der Datenerfassung
2019
Titel
Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation.
Sub types
  • research-article
  • Journal Article
Ausgabe der Zeitschrift
8

Files

https://www.tandfonline.com/doi/pdf/10.1080/20013078.2019.1615820?needAccess=true https://europepmc.org/articles/PMC6542154?pdf=render

Datenquelle: Europe PubMed Central

Abstract
Physical activity initiates a wide range of multi-systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise-associated adaptive systemic signalling. Circulating EVs comprise a heterogeneous collection of different EV-subclasses released from various cell types. So far, a comprehensive picture of the parental and target cell types, EV-subpopulation diversity and functional properties of EVs released during exercise (ExerVs) is lacking. Here, we performed a detailed EV-phenotyping analysis to explore the cellular origin and potential subtypes of ExerVs. Healthy male athletes were subjected to an incremental cycling test until exhaustion and blood was drawn before, during, and immediately after the test. Analysis of total blood plasma by EV Array suggested endothelial and leukocyte characteristics of ExerVs. We further purified ExerVs from plasma by size exclusion chromatography as well as CD9-, CD63- or CD81-immunobead isolation to examine ExerV-subclass dynamics. EV-marker analysis demonstrated increasing EV-levels during cycling exercise, with highest levels at peak exercise in all EV-subclasses analysed. Phenotyping of ExerVs using a multiplexed flow-cytometry platform revealed a pattern of cell surface markers associated with ExerVs and identified lymphocytes (CD4, CD8), monocytes (CD14), platelets (CD41, CD42, CD62P), endothelial cells (CD105, CD146) and antigen presenting cells (MHC-II) as ExerV-parental cells. We conclude that multiple cell types associated with the circulatory system contribute to a pool of heterogeneous ExerVs, which may be involved in exercise-related signalling mechanisms and tissue crosstalk.
Date of acceptance
2019
Autoren
  • Alexandra Brahmer
  • Elmo Neuberger
  • Leona Esch-Heisser
  • Nils Haller
  • Malene Moeller Jorgensen
  • Rikke Baek
  • Wiebke Möbius
  • Perikles Simon
  • Eva-Maria Krämer-Albers
Autoren-URL
https://www.ncbi.nlm.nih.gov/pubmed/31191831
DOI
10.1080/20013078.2019.1615820
Externe Identifier
  • PubMed Central ID: PMC6542154
ISSN
2001-3078
Ausgabe der Veröffentlichung
1
Zeitschrift
J Extracell Vesicles
Schlüsselwörter
  • Extracellular vesicles
  • exercise
  • exosomes
  • immunobead isolation
  • multiplex phenotyping
  • plasma
  • size exclusion chromatography
Sprache
eng
Country
United States
Paginierung
1615820
PII
1615820
Datum der Veröffentlichung
2019
Status
Published online
Titel
Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation.
Sub types
  • Journal Article
Ausgabe der Zeitschrift
8

Datenquelle: PubMed

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