Use of SNP-Arrays for ChIP Assays: Computational Aspects
- Publikationstyp:
- Kapitel
- Metadaten:
-
- Autoren
- Enrique M Muro
- Jennifer A McCann
- Michael A Rudnicki
- Miguel A Andrade-Navarro
- DOI
- 10.1007/978-1-60327-414-2_10
- ISBN-13
- 9781603274135
- Online publication date
- 2009
- Paginierung
- 145 - 154
- Buchtitel
- Chromatin Immunoprecipitation Assays
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Herausgeber
- Humana Press
- Herausgeber URL
- http://dx.doi.org/10.1007/978-1-60327-414-2_10
- Datum der Datenerfassung
- 2019
- Titel
- Use of SNP-Arrays for ChIP Assays: Computational Aspects
Datenquelle: Crossref
- Andere Metadatenquellen:
-
- Abstract
- The simultaneous genotyping of thousands of single nucleotide polymorphisms (SNPs) in a genome using SNP-Arrays is a very important tool that is revolutionizing genetics and molecular biology. We expanded the utility of this technique by using it following chromatin immunoprecipitation (ChIP) to assess the multiple genomic locations protected by a protein complex recognized by an antibody. The power of this technique is illustrated through an analysis of the changes in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during differentiation of human myoblasts into myotubes. The findings have been validated by the observation of a significant correlation between the detected histone modifications and the expression of the nearby genes, as measured by DNA expression microarrays. This chapter focuses on the computational analysis of the data.
- Addresses
- Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
- Autoren
- Enrique M Muro
- Jennifer A McCann
- Michael A Rudnicki
- Miguel A Andrade-Navarro
- DOI
- 10.1007/978-1-60327-414-2_10
- Open access
- false
- Schlüsselwörter
- Humans
- Oligonucleotide Array Sequence Analysis
- Chromatin Immunoprecipitation
- Computational Biology
- Polymorphism, Single Nucleotide
- Models, Biological
- Internet
- Software
- Programming Languages
- Databases, Genetic
- Medium
- Paginierung
- 145 - 154
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum der Datenerfassung
- 2009
- Titel
- Use of SNP-arrays for ChIP assays: computational aspects.
- Ausgabe der Zeitschrift
- 567
Datenquelle: Europe PubMed Central
- Abstract
- The simultaneous genotyping of thousands of single nucleotide polymorphisms (SNPs) in a genome using SNP-Arrays is a very important tool that is revolutionizing genetics and molecular biology. We expanded the utility of this technique by using it following chromatin immunoprecipitation (ChIP) to assess the multiple genomic locations protected by a protein complex recognized by an antibody. The power of this technique is illustrated through an analysis of the changes in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during differentiation of human myoblasts into myotubes. The findings have been validated by the observation of a significant correlation between the detected histone modifications and the expression of the nearby genes, as measured by DNA expression microarrays. This chapter focuses on the computational analysis of the data.
- Autoren
- Enrique M Muro
- Jennifer A McCann
- Michael A Rudnicki
- Miguel A Andrade-Navarro
- Autoren-URL
- https://www.ncbi.nlm.nih.gov/pubmed/19588091
- DOI
- 10.1007/978-1-60327-414-2_10
- Schlüsselwörter
- Chromatin Immunoprecipitation
- Computational Biology
- Databases, Genetic
- Humans
- Internet
- Models, Biological
- Oligonucleotide Array Sequence Analysis
- Polymorphism, Single Nucleotide
- Programming Languages
- Software
- Paginierung
- 145 - 154
- Datum der Veröffentlichung
- 2009
- Status
- Published
- Datum, an dem der Datensatz öffentlich gemacht wurde
- 2010
- Titel
- Use of SNP-arrays for ChIP assays: computational aspects.
- Ausgabe der Zeitschrift
- 567
Datenquelle: PubMed
- Beziehungen:
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